2006•Food ScienceRequires access

Study on the Use of RP-HPLC in Analysis of Aloe Polysaccharide Composition

Zhongfu Wang

Abstract

In this article, Aloe polysaccharide was extracted by water and precipitated by ethanol. The polysaccharide was hydrolyzed by TFA. A method for the derivatization of polysaccharide hydrolysate with 1-phenyl-3-methyl- 5-pyrazolone (PMP) was used to study the composition and molar ratio of three different species of Aloe by RP-HPLC. Results showed that the method of using RP-HPLC on studing Aloe polysaccharide composition is simple and convienient, and the Aloe polysaccharidescontent and sugar composition show differences among different species. Thus,a new method to study Aloe polysaccharide composition is established.

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Key concepts: Polysaccharide, Chemistry, Chromatography, Derivatization, Hydrolysate, Composition (language), High-performance liquid chromatography, Sugar