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ISOLATION,PURIFICATION AND PROPERTY STUDY OF POLYSACCHARIDE FROM ALOE

Jun Wang

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Abstract

In this article,polysaccharide in Aloe gel was extracted by water and precipitated by ethanol.It was isolated and purified by chromatography of DEAE cellulose and Sephadex G 100 columns.Three kinds of polysaccharide(AⅠ,AⅡ and AⅢ)were obtained.The results of chromatography of Sephadex G 100 column chromatography showed that AⅠ was uniform.and the molecular weight of AⅠ is 3 8×10 4.The compositions of crude and AⅠ polysaccharide were determined and quantified by GC.In addition,a majority of Aloe polysaccharide is pyranopolysaccharide.

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What this paper is about

In this article,polysaccharide in Aloe gel was extracted by water and precipitated by ethanol.It was isolated and purified by chromatography of DEAE cellulose and Sephadex G 100 columns.Three kinds of polysaccharide(AⅠ,AⅡ and AⅢ)were obtained.The results of chromatography of Sephadex G 100 column chromatography showed that AⅠ was uniform.and the molecular weight of AⅠ is 3 8×10 4.The compositions of crude and AⅠ polysaccharide were determined and quantified by GC.In addition,a majority of Aloe polysaccharide is pyranopolysaccharide.

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Available abstract

In this article,polysaccharide in Aloe gel was extracted by water and precipitated by ethanol.It was isolated and purified by chromatography of DEAE cellulose and Sephadex G 100 columns.Three kinds of polysaccharide(AⅠ,AⅡ and AⅢ)were obtained.The results of chromatography of Sephadex G 100 column chromatography showed that AⅠ was uniform.and the molecular weight of AⅠ is 3 8×10 4.The compositions of crude and AⅠ polysaccharide were determined and quantified by GC.In addition,a majority of Aloe polysaccharide is pyranopolysaccharide.

Key concepts: Polysaccharide, Sephadex, Chromatography, Chemistry, Column chromatography, Cellulose, Size-exclusion chromatography, Ethanol

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