Effect of azone and menthol on the in vitro percutaneous permeability of the compound asiaticoside gel

OBJECTIVE:To study the effect of the different concentration of azone and menthol on the percutaneous permeability of the compound Asiaticoside gel in vitro.To screen the effective transdermal absorption enhancer.METHODS:Isolated rat skin was taken as the permeable barrier by using a modified Franz absorbing-cell device.The content of Asiaticoside in the modified Franz absorbing-cell device was dertermined by HPLC,and the cumulation of Asiaticoside permeated through the rat skin was calculated.RESULTS:After adding the penetration enhancer,the penetrating rate of Asiaticoside Gel preparations increased remarkably.Menthol(1.0%0.5%2.0%),azone(1.0%4.0%2.0%),azonetwo kinds of penetration enhancers used in combinationmenthol.1.0% azone transdermal was the best.CONCLUSION:Asiaticoside gel able to obtain a higher penetration rate when the gel containing 1.0% azone.