Effect of different percutaneous absorption enhancers on the in vitro percutaneous permeability of the compound asiaticoside cataplasm

OBJECTIVE To prepare compound asiaticoside cataplasm and study the effect of different percutaneous absorption enhancer on its in vitro percutaneous permeability.METHODS A modified Franz diffusion cell device was used to study the effect of 1% azone,0.5% menthol,1% 1,2-di-hydroxypropane and 1% azone combined with 1% 1,2-di-hydroxypropane on the in vitro percutaneous permeability of the compound asiaticoside cataplasm.The contents of aisaticoside and triamcinolone acetonide acetate in the modified Franz diffusion cell device were determined by HPLC.RESULTS Transdermal diffusion in vitro of asiaticoside complied with the Higuchi equation.Transdermal diffusion in vitro of triamcinolone acetonide acetate complied with zero order equation.The four penetration enhancers could increase the permeability of the drug.The order of enhancement for asiaticoside was 1% azone combined with 1% 1,2-di-hydroxypropane 1% azone 0.5% menthol1% 1,2-di-hydroxypropane.The order of enhancement for triamcinolone acetonide acetate was 1% azone combined with 1% 1,2-di-hydroxypropane 1% azone 1% 1,2-di-hydroxypropane0.5% menthol.CONCLUSION 1% azone combined with 1% 1,2-di-hydroxypropane could be used as penetration enhancer of compound asiaticoside cataplasm.