Determination of Rutin from Buckwheat by Microcolumn High Performance Liquid Chromatography

Determination of rutin from buckwheat (fagopyrum tataricum) by microcolumn high performance liquid chromatography was studied. Rutin was extracted from samples with 80% of methanol by heating for 30min and isolated on a Waters Xterra TM RP-18 chromatographic column (1.0×50?mm, 2.5?μm) eluted with methanol containing 1% of acetic acid at a flow rate of 0.2?ml/min, and monitored with the photodiode array detector at 356?nm. The method was applied to the determination of rutin from buckwheat with good results.