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2004•Journal of Yunnan University for NationalitesRequires access

Study on the determination of rutin in hupericum perforatum with microcolumn high performance liquid chromatography

Qiufen Hu

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Abstract

A microcolumn HPLC method for the determination of rutin in hupericum perforatum was studied. The rutin was extracted from the sample with 80% of methanol, then was separated on a Waters Xterra~TM RP_18 (1.0×30?mm, 2.5?μm) chromatographic column, with methanol and 1% acetic acid solution as mobile phase. The detection wavelength is 356?nm. This method was applied to the determination of rutin in hupericum perforatum with good results.

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A microcolumn HPLC method for the determination of rutin in hupericum perforatum was studied. The rutin was extracted from the sample with 80% of methanol, then was separated on a Waters Xterra~TM RP_18 (1.0×30?mm, 2.5?μm) chromatographic column, with methanol and 1% acetic acid solution as mobile phase. The detection wavelength is 356?nm. This method was applied to the determination of rutin in hupericum perforatum with good results.

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Available abstract

A microcolumn HPLC method for the determination of rutin in hupericum perforatum was studied. The rutin was extracted from the sample with 80% of methanol, then was separated on a Waters Xterra~TM RP_18 (1.0×30?mm, 2.5?μm) chromatographic column, with methanol and 1% acetic acid solution as mobile phase. The detection wavelength is 356?nm. This method was applied to the determination of rutin in hupericum perforatum with good results.

Key concepts: Rutin, Chromatography, Chemistry, High-performance liquid chromatography, Acetic acid, Methanol, Organic chemistry, Antioxidant

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