Effects of Different Plant Hormone Combination on Bud Inducement of Seedless Water Melons

[Objective] The aim was to explore tissue culture technique of Motong seedless watermelon.[Method] Cotyledon of seedless watermelon was made as the explant.The explants were cultured on MS medium with different concentrations of 6-BA,IAA,IBA and KT for inducing callus,clumpy buds and clumpy bud enlongation.[Result] The concentrations from 0.3 mg/L to 1.0 mg/L of IAA,6-BA are able to induce callus;the concentrations from 0.6 to 2.5 mg/L 6-BA,IBA could induce bud;the concentrations from 0.2 to 1.0 mg/L KT could induce shoot elongation.But there were many differences.[Conclusion] The results showed that the best hormone combination of inducing callus was MS+NAA 1.0 mg/L+6-BA 0.5 mg/L.The best hormone combination of inducing clumpy buds was MS+6-BA 2.0 mg/L+IBA 0.8 mg/L.The best hormone combination of clumpy bud enlongation was MS+KT 0.2 mg/L.