2009•Journal of Molecular Diagnostics and TherapyRequires access

Early diagnosis of mycoplasma pneumonia infection in children by PCR technique

Fen Xiaosan

Abstract

Objective To explore the early diagnosis of mycoplasma pneumonia(MP) infection in Children. Methods Fluorescence quantitative polymerase chain reaction(FQ-PCR)technique were adopted respectively to detect the DNA of MP and mycoplasma pneumonia IgM antibody in serum samples in 1～10 infants with the prescribed diagnosis of respiratory tract infection. Results The positive rate in the results between MP-DNA and IgM antibody was statistically significant, In one to three years old children group(P0.05). Conclusion MP is one of important pathogens that cause respiratory tract infection of children.FQ-PCR method is a rapid,sensitive and specific assessment in detecting the MP-DNA.

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Key concepts: Mycoplasma pneumonia, Mycoplasma pneumoniae, Pneumonia, Mycoplasma, Respiratory tract, Polymerase chain reaction, Respiratory tract infections, Antibody