Determination of β-asarone in Shenqiyinao Capsules by HPLC

ObjectiveTo establish a high performance liquid chromatographic method for the determination of β-asarone in Shenqiyinao Capsules.Methods The column was Thermo BDS Hypersil C18(250 mm×4.6 mm,5 μm).The mobile phase was methanol-0.1% glacial acetic acid solution(60∶40) and the temperature of column was 35 ℃.The flow rate was 1.0 ml·min-1 and the detection wavelength was 257 nm with external method.Results The linear range of β-asarone was 4.885 ～ 146.55 μg·ml-1.The average recoveries of β-asarone were 103.2%,102.1% and 100.2% with corresponding RSD of 0.4%,0.3% and 0.7%,respectivery.Conclusion The method is simple,sensitive,accurate and can be used for the determination of Rhizoma Acori Tatarinowii(RAT) in Shenqiyinao Capsules.