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2004•The Chinese Journal of Modern Applied PharmacyRequires access

Determination of paeoniflorin in Xiaoyukang capsules by HPLC

LI Sheng-you

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Abstract

OBJECTIVE To establish a HPLC method for determination of paeoniflorin in Xiaoyukang capsules. METHOD Using YWG-C 18 column(4.6mm×150mm),the mobile phase was methanol-water(22∶78), at flow rate of 1.0mL·min -1, the UV detection wavelength was 230nm,the column temperature was 30℃.RESULTS The average recovery was 99.3% RSD was 1.01%. The RSD of the repeatability test was 1.42%(n=5). CONCLUSION The method is simple and accurate and can be used for the quality control of Xiaoyukang capsules.

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What this paper is about

OBJECTIVE To establish a HPLC method for determination of paeoniflorin in Xiaoyukang capsules. METHOD Using YWG-C 18 column(4.6mm×150mm),the mobile phase was methanol-water(22∶78), at flow rate of 1.0mL·min -1, the UV detection wavelength was 230nm,the column temperature was 30℃.RESULTS The average recovery was 99.3% RSD was 1.01%. The RSD of the repeatability test was 1.42%(n=5). CONCLUSION The method is simple and accurate and can be used for the quality control of Xiaoyukang capsules.

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Available abstract

OBJECTIVE To establish a HPLC method for determination of paeoniflorin in Xiaoyukang capsules. METHOD Using YWG-C 18 column(4.6mm×150mm),the mobile phase was methanol-water(22∶78), at flow rate of 1.0mL·min -1, the UV detection wavelength was 230nm,the column temperature was 30℃.RESULTS The average recovery was 99.3% RSD was 1.01%. The RSD of the repeatability test was 1.42%(n=5). CONCLUSION The method is simple and accurate and can be used for the quality control of Xiaoyukang capsules.

Key concepts: Paeoniflorin, Chromatography, Repeatability, High-performance liquid chromatography, Chemistry

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