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Progress on Extraction Methods of DNA from Cotton

Yanhua Tan

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Abstract

Cotton was one of important economic crops in China.Because of the rich gossypol,polysaccharides,tannins and other interfering compounds in cotton,the extraction and purification of DNA were much more difficult than other crops.The extraction methods of cotton's genomic DNA were reviewed.In these extraction methods,SDS method and CTAB method were commonly used,and the CTAB method and its modified method resulted in best extraction outcome.

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What this paper is about

Cotton was one of important economic crops in China.Because of the rich gossypol,polysaccharides,tannins and other interfering compounds in cotton,the extraction and purification of DNA were much more difficult than other crops.The extraction methods of cotton's genomic DNA were reviewed.In these extraction methods,SDS method and CTAB method were commonly used,and the CTAB method and its modified method resulted in best extraction outcome.

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Available abstract

Cotton was one of important economic crops in China.Because of the rich gossypol,polysaccharides,tannins and other interfering compounds in cotton,the extraction and purification of DNA were much more difficult than other crops.The extraction methods of cotton's genomic DNA were reviewed.In these extraction methods,SDS method and CTAB method were commonly used,and the CTAB method and its modified method resulted in best extraction outcome.

Key concepts: Extraction (chemistry), DNA extraction, DNA, Gossypol, genomic DNA, Chromatography, Polysaccharide, Biotechnology

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