Extraction of Total RNA in Cotton Tissues with CTAB-acidic Phenolic Method
Jiang Jian
Abstract
Jiang Jian
Abstract
Based on the CTAB extraction method for cotton total DNA and the LiCl precipitation method of RNA, a new method suitable for cotton total RNA extraction, named CTAB acidic pheonlic method, was come into being. The method could produce cotton total RNA with higher purity and integrality in a shorter time from different tissues of cotton than other conventional methods.
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Based on the CTAB extraction method for cotton total DNA and the LiCl precipitation method of RNA, a new method suitable for cotton total RNA extraction, named CTAB acidic pheonlic method, was come into being. The method could produce cotton total RNA with higher purity and integrality in a shorter time from different tissues of cotton than other conventional methods.
Key concepts: Extraction (chemistry), RNA, RNA extraction, Chromatography, Chemistry, DNA, Precipitation, Biochemistry