Research on Soluble Protein Changes in Several Types of Wheat Male Sterile Line during the Fertility-sensitive Period

[Objective] To study the soluble protein changes in several types of wheat male sterile line during the fertility-sensitive period.[Method] Two K-types wheat male sterile lines,the maintainer line and the YS type temperature sensitive male sterile line were selected as the research objects,their soluble protein contents of the penultimate leaf and anther were studied in the process of pollen development.[Result] Protein content in the male sterile line anther tended to increase from meiosis to uninucleate stage,declined from uninucleate stage to trinucleate stage,and the descent rate was the fastest between uninucleate stage and binucleate stage among all stages.The protein content of maintainer line from uninucleate stage to trinucleate stage declined slower than that of the male sterile line,the soluble protein content of the male sterile line was significantly lower than that of the maintainer lines during the entire period.Soluble protein content of anther had more change than that of leaves of sterile line and maintainer line during the whole growth period.Protein content had no significant difference between the male sterile line and YS type temperature sensitive male sterile line.Soluble protein content of the male sterile line was found decreasing significantly.[Conclusion] Protein synthesis blocked or more protein degradation will lead to physiological metabolism disorders,and the development of pollen will be hindered.