ScholarLens Back to search
2009•Food Science and Technology InternationalRequires access

Determination of Oleanolic acid in Achyranthes bientata B1. with RP-HPLC

Hong San-guo

Open publisher page 0 citations

Abstract

Objective:To establish an RP -HPLC method to determine the content of oleanolic acid in Achyranthes bientata B1.. Methods: By ultrasonic extraction technique, the suitable chromatogram conditions: Allsphere ODS-2 column(250 mm×4.6 mm, 5 μ), the mobile phase was methanol-water-acetic acid(80∶20∶0.01, v/v/v), column temperature was at 30 ℃, flow rate was 1.0 mL/min, UV detection wavelength was 215 nm. Results: The good linear range of Oleanolic Acid was 0.01～0.4 mg/mL, regression equation: Y=2796327X-27842, r=0.9997, the average recovery rate was 98.96%, RSD=0.21%(n=5). The content of Oleanolic acid is the highest in Shandong Province, in comparison of others. Conclusion: The method is simple, accurate and can be used to evaluate the quality of Achyranthes bientata B1.

About this research paper

What this paper is about

Objective:To establish an RP -HPLC method to determine the content of oleanolic acid in Achyranthes bientata B1.. Methods: By ultrasonic extraction technique, the suitable chromatogram conditions: Allsphere ODS-2 column(250 mm×4.6 mm, 5 μ), the mobile phase was methanol-water-acetic acid(80∶20∶0.01, v/v/v), column temperature was at 30 ℃, flow rate was 1.0 mL/min, UV detection wavelength was 215 nm. Results: The good linear range of Oleanolic Acid was 0.01～0.4 mg/mL, regression equation: Y=2796327X-27842, r=0.9997, the average recovery rate was 98.96%, RSD=0.21%(n=5). The content of Oleanolic acid is the highest in Shandong Province, in comparison of others. Conclusion: The method is simple, accurate and can be used to evaluate the quality of Achyranthes bientata B1.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Objective:To establish an RP -HPLC method to determine the content of oleanolic acid in Achyranthes bientata B1.. Methods: By ultrasonic extraction technique, the suitable chromatogram conditions: Allsphere ODS-2 column(250 mm×4.6 mm, 5 μ), the mobile phase was methanol-water-acetic acid(80∶20∶0.01, v/v/v), column temperature was at 30 ℃, flow rate was 1.0 mL/min, UV detection wavelength was 215 nm. Results: The good linear range of Oleanolic Acid was 0.01～0.4 mg/mL, regression equation: Y=2796327X-27842, r=0.9997, the average recovery rate was 98.96%, RSD=0.21%(n=5). The content of Oleanolic acid is the highest in Shandong Province, in comparison of others. Conclusion: The method is simple, accurate and can be used to evaluate the quality of Achyranthes bientata B1.

Key concepts: Oleanolic acid, Chromatography, Chemistry, Content determination, High-performance liquid chromatography, Acetic acid, Recovery rate, Methanol

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Determination of Oleanolic acid in Achyranthes bientata B1. with RP-HPLC — Research Paper | ScholarLens