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HPLC determination of oleanolic acid in achyranthes produced in Sichuan

Zhuang Yuan-chun

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Abstract

Objective:To establish a HPLC method for the determination of oleanolic acid in achyranthes produced in Sichuan.Methods:The separation was carried out on the Dikma Kromasil C18 column(250 mm×4.6 mm,5 μm).The mobile phase was methanol-water(92:8).The detective wavelength was set at 210nm.The column temperature was 30℃ and the flow rate was 1.0 mL·min-1 with the sample volume of 10μL.Results:The calibration curve showed good linearity over the range of 1-10 μg(r=0.9999).The average recovery rate was 96.97% with RSD 1.02%.Conclusion:This method is simple,rapid,accurate with good repeatability and good recovery,which can be used as the quantitative analysis method of oleanolic acid for achyranthes produced in Sichuan.

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What this paper is about

Objective:To establish a HPLC method for the determination of oleanolic acid in achyranthes produced in Sichuan.Methods:The separation was carried out on the Dikma Kromasil C18 column(250 mm×4.6 mm,5 μm).The mobile phase was methanol-water(92:8).The detective wavelength was set at 210nm.The column temperature was 30℃ and the flow rate was 1.0 mL·min-1 with the sample volume of 10μL.Results:The calibration curve showed good linearity over the range of 1-10 μg(r=0.9999).The average recovery rate was 96.97% with RSD 1.02%.Conclusion:This method is simple,rapid,accurate with good repeatability and good recovery,which can be used as the quantitative analysis method of oleanolic acid for achyranthes produced in Sichuan.

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Available abstract

Objective:To establish a HPLC method for the determination of oleanolic acid in achyranthes produced in Sichuan.Methods:The separation was carried out on the Dikma Kromasil C18 column(250 mm×4.6 mm,5 μm).The mobile phase was methanol-water(92:8).The detective wavelength was set at 210nm.The column temperature was 30℃ and the flow rate was 1.0 mL·min-1 with the sample volume of 10μL.Results:The calibration curve showed good linearity over the range of 1-10 μg(r=0.9999).The average recovery rate was 96.97% with RSD 1.02%.Conclusion:This method is simple,rapid,accurate with good repeatability and good recovery,which can be used as the quantitative analysis method of oleanolic acid for achyranthes produced in Sichuan.

Key concepts: Oleanolic acid, Repeatability, Medicine, Chromatography, High-performance liquid chromatography, Content determination, Calibration curve, Detection limit

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