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Determination of Content of Caffeic Acid in Manpenyihao Powder by HPLC

Mei Quanxi

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Abstract

Objective To establish a method for the content determination of caffeic acid in manpenyihao powder.Methods The C18 was used as column,with mathanol-0.01 mol/L sodium dihydrgen phosphate solution(pH=4.0,20∶80) as the mobile phase at a flow rate of 1 ml/min.The detection wavelength was at 323 nm.The column temperature was 40℃,and the injection volumn was 10 μl.Results The linear range of caffeic acid was 0.040 12~0.240 7 μg(r=0.999 5).The average recovery was 100.2%(RSD=0.7%).Conclusion The method is simple,accurate,and special.It can be used for the quality control of manpenyihao powder.

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What this paper is about

Objective To establish a method for the content determination of caffeic acid in manpenyihao powder.Methods The C18 was used as column,with mathanol-0.01 mol/L sodium dihydrgen phosphate solution(pH=4.0,20∶80) as the mobile phase at a flow rate of 1 ml/min.The detection wavelength was at 323 nm.The column temperature was 40℃,and the injection volumn was 10 μl.Results The linear range of caffeic acid was 0.040 12~0.240 7 μg(r=0.999 5).The average recovery was 100.2%(RSD=0.7%).Conclusion The method is simple,accurate,and special.It can be used for the quality control of manpenyihao powder.

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Available abstract

Objective To establish a method for the content determination of caffeic acid in manpenyihao powder.Methods The C18 was used as column,with mathanol-0.01 mol/L sodium dihydrgen phosphate solution(pH=4.0,20∶80) as the mobile phase at a flow rate of 1 ml/min.The detection wavelength was at 323 nm.The column temperature was 40℃,and the injection volumn was 10 μl.Results The linear range of caffeic acid was 0.040 12~0.240 7 μg(r=0.999 5).The average recovery was 100.2%(RSD=0.7%).Conclusion The method is simple,accurate,and special.It can be used for the quality control of manpenyihao powder.

Key concepts: Caffeic acid, Chromatography, Linear range, High-performance liquid chromatography, Chemistry, Content determination, Phosphate buffered saline, Detection limit

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Determination of Content of Caffeic Acid in Manpenyihao Powder by HPLC — Research Paper | ScholarLens