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2007•Unpublished venueRequires access

Determination of caffeic acid in Qingyanning spray by HPLC

Yang Xiong-zhi

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Abstract

Objective To establish an HPLC method for determination of caffeic acid in Qingyanning spray.Methods The sample was analyzed on a BDS C18 column(200 mm×5.0 mm,5μm) using methanol-0.013 mol/L phosphate salt solution(23∶77) as the mobile phase.The flow rate was 1.0 mL/min and the detection wavelength 320 nm.Results The method exhibited good linearity from 0.042 5 to 0.425 μg(r=0.999 9).The average recovery was 99.05% and RSD 2.9%.Conclusion The method is convenient,reliable and accurate for the quality control of the spray.

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What this paper is about

Objective To establish an HPLC method for determination of caffeic acid in Qingyanning spray.Methods The sample was analyzed on a BDS C18 column(200 mm×5.0 mm,5μm) using methanol-0.013 mol/L phosphate salt solution(23∶77) as the mobile phase.The flow rate was 1.0 mL/min and the detection wavelength 320 nm.Results The method exhibited good linearity from 0.042 5 to 0.425 μg(r=0.999 9).The average recovery was 99.05% and RSD 2.9%.Conclusion The method is convenient,reliable and accurate for the quality control of the spray.

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Available abstract

Objective To establish an HPLC method for determination of caffeic acid in Qingyanning spray.Methods The sample was analyzed on a BDS C18 column(200 mm×5.0 mm,5μm) using methanol-0.013 mol/L phosphate salt solution(23∶77) as the mobile phase.The flow rate was 1.0 mL/min and the detection wavelength 320 nm.Results The method exhibited good linearity from 0.042 5 to 0.425 μg(r=0.999 9).The average recovery was 99.05% and RSD 2.9%.Conclusion The method is convenient,reliable and accurate for the quality control of the spray.

Key concepts: Chromatography, Caffeic acid, Chemistry, High-performance liquid chromatography, Phosphate buffered saline, Methanol, Antioxidant, Biochemistry

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