2010•Zhongguo shiyan fangjixue zazhiRequires access

Determination of Caffeic Acid in PYJP by HPLC

HE Sheng-li

Abstract

Objective:To develop an HPLC method for determination of the content of caffeic acid in PYJP.Method:An C18 column was used as the chromatographic column.The mobile phase consisted of methanol-phosphate buffer(23∶ 77),the flow rate was 1.0 mL.min-1.The UV detection wavelength was at 323 nm.Result:There was a good linear relationship between the concentration of caffeic acid and peak area value when the concentrations of caffeic acid was within the range of 0.072 96-0.437 76 μg(r = 0.999 9).The average recovery was 99.10%,RSD 1.22%(n = 5).Conclusion:The method was accurate,sensitive,reproducible and may be used in the determina-tion of caffeic acid in PYJP

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Key concepts: Caffeic acid, Chromatography, High-performance liquid chromatography, Chemistry, Phosphate buffered saline, Linear range, Methanol, Linear relationship