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2008•Zhongguo zhongyiyao xinxi zazhiRequires access

Determination of the Content of Caffeic Acid in Chanhou Tieshijin by HPLC

Ling Bian

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Abstract

Objective To establish HPLC method for the determination of the content of caffeic acid in Chanhou Tieshijin. Methods The determination was performed on HangBang C18 column (200 mm×4.6 mm, 5 μm). The mobile phase was methanol-buffer phosphate (23∶77) at a flow rate of 1.0 mL/min. The UV detection wavelength was set at 323 nm and the sample size was 10 μL. Results The linear range of caffeic acid was 0.052～0.26 μg (r=0.999 8), with average recovery rate of 97.44% (RSD=0.78%). Conclusion The established method is simple, rapid and accurate, and suitable for the quality control of Chanhou Tieshijin.

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What this paper is about

Objective To establish HPLC method for the determination of the content of caffeic acid in Chanhou Tieshijin. Methods The determination was performed on HangBang C18 column (200 mm×4.6 mm, 5 μm). The mobile phase was methanol-buffer phosphate (23∶77) at a flow rate of 1.0 mL/min. The UV detection wavelength was set at 323 nm and the sample size was 10 μL. Results The linear range of caffeic acid was 0.052～0.26 μg (r=0.999 8), with average recovery rate of 97.44% (RSD=0.78%). Conclusion The established method is simple, rapid and accurate, and suitable for the quality control of Chanhou Tieshijin.

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Available abstract

Objective To establish HPLC method for the determination of the content of caffeic acid in Chanhou Tieshijin. Methods The determination was performed on HangBang C18 column (200 mm×4.6 mm, 5 μm). The mobile phase was methanol-buffer phosphate (23∶77) at a flow rate of 1.0 mL/min. The UV detection wavelength was set at 323 nm and the sample size was 10 μL. Results The linear range of caffeic acid was 0.052～0.26 μg (r=0.999 8), with average recovery rate of 97.44% (RSD=0.78%). Conclusion The established method is simple, rapid and accurate, and suitable for the quality control of Chanhou Tieshijin.

Key concepts: Caffeic acid, Chromatography, Content determination, High-performance liquid chromatography, Chemistry, Linear range, Phosphate buffered saline, Recovery rate

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