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HPLC determination of hesperidin in Fuyankangfu tablets

Yujie Zhang

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Abstract

Objective:To establish the content determination method of hesperidin Fuyankangfu tablets.Methods: HPLC analysis was carried out with C_(18)(250 mm×4.6 mm,5 μm)column and methanol-glacial acetic acid-wa- ter(35:4:61)as the mobile phase.The flow rate was 1.0 mL·min~(-1);the column temperature was 25℃;the de- tection wavelength was 283 nm.Results:The calibrattion curves were linear in the range of 0.36-2.16 μg· mL~(-1).The regression equation was A=90.24C+111.96,r=0.9985(n=6).The average recovery was 101.4%(n =3).Conclusion:The method is simple and sensitive.It can be used for the determination of hesperidin in Fuyan- kangfu tablets.

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What this paper is about

Objective:To establish the content determination method of hesperidin Fuyankangfu tablets.Methods: HPLC analysis was carried out with C_(18)(250 mm×4.6 mm,5 μm)column and methanol-glacial acetic acid-wa- ter(35:4:61)as the mobile phase.The flow rate was 1.0 mL·min~(-1);the column temperature was 25℃;the de- tection wavelength was 283 nm.Results:The calibrattion curves were linear in the range of 0.36-2.16 μg· mL~(-1).The regression equation was A=90.24C+111.96,r=0.9985(n=6).The average recovery was 101.4%(n =3).Conclusion:The method is simple and sensitive.It can be used for the determination of hesperidin in Fuyan- kangfu tablets.

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Available abstract

Objective:To establish the content determination method of hesperidin Fuyankangfu tablets.Methods: HPLC analysis was carried out with C_(18)(250 mm×4.6 mm,5 μm)column and methanol-glacial acetic acid-wa- ter(35:4:61)as the mobile phase.The flow rate was 1.0 mL·min~(-1);the column temperature was 25℃;the de- tection wavelength was 283 nm.Results:The calibrattion curves were linear in the range of 0.36-2.16 μg· mL~(-1).The regression equation was A=90.24C+111.96,r=0.9985(n=6).The average recovery was 101.4%(n =3).Conclusion:The method is simple and sensitive.It can be used for the determination of hesperidin in Fuyan- kangfu tablets.

Key concepts: Hesperidin, Chemistry, Chromatography, High-performance liquid chromatography, Acetic acid, Methanol, Analytical Chemistry (journal), Alternative medicine

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