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Determination of Astragaloside IV in Radix Astragali by SPE-HPLC

Shungang Wan

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Abstract

Objective To establish SPEHPLC method for the determination of astragaloside IV in Radix Astragali.Methods The astragaloside IV in sample was purified with C_(18) solid phase column and its content was determined with HPLC-UV method.The astragaloside IV was separated on a Zorbax SB-C_(18) column(φ4.6 mm×150 mm,5 μm) with acetonitrile-water(32∶68,v/v) as mobile phase,and the detective wavelength was set at 200nm.Results The linear range was 1.408～7.04 μg(r=0.999 9).The average recovery of astragaloside IV was 101.5%.Conclusion The method is simple,accurate and can be used for the quality control of Radix Astragali and its preparation.

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Objective To establish SPEHPLC method for the determination of astragaloside IV in Radix Astragali.Methods The astragaloside IV in sample was purified with C_(18) solid phase column and its content was determined with HPLC-UV method.The astragaloside IV was separated on a Zorbax SB-C_(18) column(φ4.6 mm×150 mm,5 μm) with acetonitrile-water(32∶68,v/v) as mobile phase,and the detective wavelength was set at 200nm.Results The linear range was 1.408～7.04 μg(r=0.999 9).The average recovery of astragaloside IV was 101.5%.Conclusion The method is simple,accurate and can be used for the quality control of Radix Astragali and its preparation.

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Available abstract

Objective To establish SPEHPLC method for the determination of astragaloside IV in Radix Astragali.Methods The astragaloside IV in sample was purified with C_(18) solid phase column and its content was determined with HPLC-UV method.The astragaloside IV was separated on a Zorbax SB-C_(18) column(φ4.6 mm×150 mm,5 μm) with acetonitrile-water(32∶68,v/v) as mobile phase,and the detective wavelength was set at 200nm.Results The linear range was 1.408～7.04 μg(r=0.999 9).The average recovery of astragaloside IV was 101.5%.Conclusion The method is simple,accurate and can be used for the quality control of Radix Astragali and its preparation.

Key concepts: Astragaloside, Radix (gastropod), Chromatography, High-performance liquid chromatography, Chemistry, Content determination, Linear range, Detection limit

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