Relationship between expression of CDC6,CDK1 and HPV_(16/18) infection in uterine cervical carcinogenesis

Objective:To investigate the relationship between expression of CDC6,CDK1 and HPV16/18 infection in uterine cervical carcinogenesis.Methods:Formalin-fixed,paraffin-embedded samples including 50 cases of squamous cell carcinomas,20 cases of cervical intraepithelial neoplasia CINⅡ-Ⅲ and 20 cases of CINⅠ and 20 cases of normal cervical tissues were studied.Using polymer detection system for immunohistological staining(PV-9000) immunohistochemical technique,to examine the expression of CDC6 and CDK1 in these samples.The infections of HPV type 16,18 DNA were determined by PCR.Results:There were significant differences for CDC6,and CDK1 between carcinomas,CIN and normal cervices,(all P0.05).Expression of CDC6 and CDK1 was correlated with tumor grades(all P0.05).Expression of CDC6 was correlated with lymphnode metastasis(P0.05).Expression of CDK1 was not correlated with lymphnode metastasis(P0.05).Expressions of CDC6 and CDK1 were not correlated with age and clinical stages(all P0.05).The positive rate of HPV16/18 was significant different between cervical carcinomas,CIN and normal tissues(P0.05) and not associated with age,tumor grade,clinical stages and lymph node metastasis(P0.05).The expression of CDK1 was positively correlated with the CDC6(rs = 0.529,P0.05).The positive rate of HPV16/18 was positively correlated with CDC6(rs =0.386,P0.05).CDK1 was not correlated with HPV16/18(rs =0.145,P0.05).Conclusion:CIN and cervical carcinomas changes in CDC6 expression may be associated with HPV16/18 infection and they interact with each other,which can influent the progression of CIN and carcinogenesis of cervical carcinomas.Expressions of CDC6 andCDK1 are associated with uterine cervical carcinogenesis,they possibly become useful indexes for the primary screening and prognosis of cervical carcinomas.