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2011•Unpublished venueRequires access

Determination of protocatechuic acid in Zhidai granules by HPLC

Kaijun Lei

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Abstract

Objective To establish a HPLC method to the assay of protocatechuic acid in Zhidai granules.Methods Protocatechuic acid was determined by Hypersil C18 column(4.6 mm× 250 mm,5 μm) using methanol-water-acetic acid(19∶80∶1)(ζ)as mobile phase with the flow rate of 1.0 mL·min-1 and the detection wavelength was 260 nm.Results There was a good linear relationship between the concentration and peak area value of protocatechuic acid in the range of 0.051 2-0.768 0 μg(r=0.999 8).The average recovery was 100.90% with the RSD of 1.79%(n=6).Conclusion This method is specific,reliable,reproducible and feasible,which can be used for quality control of Zhidai granules.

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What this paper is about

Objective To establish a HPLC method to the assay of protocatechuic acid in Zhidai granules.Methods Protocatechuic acid was determined by Hypersil C18 column(4.6 mm× 250 mm,5 μm) using methanol-water-acetic acid(19∶80∶1)(ζ)as mobile phase with the flow rate of 1.0 mL·min-1 and the detection wavelength was 260 nm.Results There was a good linear relationship between the concentration and peak area value of protocatechuic acid in the range of 0.051 2-0.768 0 μg(r=0.999 8).The average recovery was 100.90% with the RSD of 1.79%(n=6).Conclusion This method is specific,reliable,reproducible and feasible,which can be used for quality control of Zhidai granules.

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Available abstract

Objective To establish a HPLC method to the assay of protocatechuic acid in Zhidai granules.Methods Protocatechuic acid was determined by Hypersil C18 column(4.6 mm× 250 mm,5 μm) using methanol-water-acetic acid(19∶80∶1)(ζ)as mobile phase with the flow rate of 1.0 mL·min-1 and the detection wavelength was 260 nm.Results There was a good linear relationship between the concentration and peak area value of protocatechuic acid in the range of 0.051 2-0.768 0 μg(r=0.999 8).The average recovery was 100.90% with the RSD of 1.79%(n=6).Conclusion This method is specific,reliable,reproducible and feasible,which can be used for quality control of Zhidai granules.

Key concepts: Protocatechuic acid, Chromatography, Chemistry, Acetic acid, High-performance liquid chromatography, Linear relationship, Linear range, Methanol

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