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2006•Zhongguo yaofangRequires access

Content Determination of Protocatochuic Acid in Fuxuekang Granules by HPLC

Zou Yan, Xie Yun

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Abstract

OBJECTIVE:To establish an HPLC method for content determination of protocatechuic acid in fuxuekang granwles.METHODS:Hypersil C 18 was used as chromatographic column,methanol-water(10∶90)as mobile phase,with a flow rate at1.0ml/min.The column temperature was room temperature,the detection wavelength at256nm and the sample size was20μl.RESULTS:The calibration curve of protocatechuic acid was linear within the range of10.2～306.0μg/ml(r=0.9999).The average recovery was99.6%(RSD=1.7%).CONCLUSION:The established method is accurate,reproducible and applicable for the content determination and quality control of fuxuekang granules.

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What this paper is about

OBJECTIVE:To establish an HPLC method for content determination of protocatechuic acid in fuxuekang granwles.METHODS:Hypersil C 18 was used as chromatographic column,methanol-water(10∶90)as mobile phase,with a flow rate at1.0ml/min.The column temperature was room temperature,the detection wavelength at256nm and the sample size was20μl.RESULTS:The calibration curve of protocatechuic acid was linear within the range of10.2～306.0μg/ml(r=0.9999).The average recovery was99.6%(RSD=1.7%).CONCLUSION:The established method is accurate,reproducible and applicable for the content determination and quality control of fuxuekang granules.

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Available abstract

OBJECTIVE:To establish an HPLC method for content determination of protocatechuic acid in fuxuekang granwles.METHODS:Hypersil C 18 was used as chromatographic column,methanol-water(10∶90)as mobile phase,with a flow rate at1.0ml/min.The column temperature was room temperature,the detection wavelength at256nm and the sample size was20μl.RESULTS:The calibration curve of protocatechuic acid was linear within the range of10.2～306.0μg/ml(r=0.9999).The average recovery was99.6%(RSD=1.7%).CONCLUSION:The established method is accurate,reproducible and applicable for the content determination and quality control of fuxuekang granules.

Key concepts: Chromatography, Calibration curve, Protocatechuic acid, Content determination, Chemistry, High-performance liquid chromatography, Linear range, Linear relationship

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Content Determination of Protocatochuic Acid in Fuxuekang Granules by HPLC — Research Paper | ScholarLens