Determination of Puerarin, Daidzin and Daidzein in Puerariae Lobatae Radix by ILDLPME Coupled with HPLC

Objective To develop a sensitive and specific HPLC method to simultaneously determine the contents of puerarin, daidzin and daidzein in Puerariae Lobatae Radix. Methods The three compounds were obtained by ionic liquid based on dispersive liquid phase microextraction. The determination was carried out on a Phenomenex C18 column(250 mm×4.6 mm, 5 μm) with a mixture of methanol-0.2% acetic acid(volume ration 45∶55) as mobile phase at a flow rate of 0.8 mL/min. The UV detective wavelength was 250 nm, and the column temperature was set at 35 ℃. Results The linear response ranged from 6.24×10-6-37.44 μg for puerarin(r =0.999 71), 5.44×10-6-27.20 μg for daidzin(r =0.999 85), and 5.60×10-6-28.00 μg for daidzein(r =0.999 94), respectively. Conclusion The method is quick, simple and repeatable for simultaneous determination of the contents of puerarin, daidzin and daidzein in Puerariae Lobatae Radix.