Content determination of puerarin and daidzein in Puerariae Lobatae Radix from different origins of Guangdong

Objective To determine the contents of puerarin and daidzein in Puerariae Lobatae Radix from different growing areas of Guangdong,and provide reference for establishing its quality standards and identifying the authentic ones.Methods The content was determined by RP-HPLC method with a Phenomenex C18column(250 mm × 4.6 mm,5 μm) eluting with gradient methanol-water at a flow rate of 1.0 mL.min-1.The column temperature was 27 ℃,and the UV detection wavelength was set at 250 nm.Results The linear range was within 0.992-39.68 μg.mL-1 for puerafin and within 0.198-7.936 μg.mL-1 for daidzein(r = 0.999 7).The average recovery of puerafin was 101.3%(RSD = 0.86%,n = 6) and daidzein was 98.0%(RSD = 1.7%,n = 6).Conclusion This method may be used for quality control and identification of Puerariae Lobatae Radix.