2011•Zhongguo zhongyiyao xinxi zazhiRequires access

Determination of Hesperidin in Xiangsha Hezhong Wan by HPLC

Cai Jun-an

Abstract

Objective To establish the method for detemining the content of Hesperidin in Xiangsha Hezhong Wan by HPLC.Methods Diamonsil ODS1 C18 column was used with methanol-acetic acid-water(35∶4∶61)as the mobile phase,detection wavelength as 283 nm,and flow rate was 1.0 mL/min.Results The calibration curve was linear at the range of 0.42-8.4μg for Hesperidin and linear equation was Y= 830 125.1X+5 849.875,r=0.999 8.The average recovery was 99.35%and the related standard deviation(RSD)was 0.68%(n=6).Conclusion This method was simple,accurate and proper,and the reduplication of the result was good.It provides quantitative analysis method for Hesperidin in Xiangsha Hezhong Wan.

About this research paper

What this paper is about

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Key concepts: Hesperidin, Chromatography, High-performance liquid chromatography, Calibration curve, Linear relationship, Acetic acid, Chemistry, Mathematics