2005•Lishizhen Medicine and Materia Medica ResearchRequires access

Determination of Paeoniflorin in Weikangling Capsule by HPLC

Liying Chang

Abstract

Objective:To establish the HPLC method for the determination of paeoniflorin in Weikangling Capsule.Methods:The chromatographic conditions:the column is Dikma ODS C(18) column(250 mm×4.6 mm),the mobile phase is methol-water, the detection wavelength is 230 nm and the column temperature is 25℃.Results:The linear range of paeoniflorin was 0.204 8～2.048 0 μg. r=0.999 9. The average recovery of paeoniflorin was 99.3% with RSD 0.93%.Conclusion:The method is accurate and can be used as a determination method of paeoniflorin in Weikangling Capsule.

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Key concepts: Paeoniflorin, Chromatography, Capsule, High-performance liquid chromatography, Chemistry, Linear range, Detection limit, Biology