2005•Tianran chanwu yanjiu yu kaifaRequires access

Determination of Ursolic Acid in Plants by HPLC

Chen Wu

Abstract

The content of ursolic acid in six plants was determined by HPLC.HPLC was carried out on a SymmetryShield RP18 column at 25 ℃ using MeOH-H 2O-H 3PO 4(88:12:0.1)as solvents with a flow rate of 1.0 mL/min detected at 210 nm.The linear range of determination were 0.192～3.072 μg,R=0.9999.The mean recovery of 98.12%,RSD 1.7%(n=5) was obtained.The method is sensitive,accurate and reproducible.

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Key concepts: Ursolic acid, Chromatography, High-performance liquid chromatography, Chemistry, Linear relationship, Linear range, Recovery rate, Detection limit