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Comparison of Two Methods of Genomic DNA Extraction from Pork Liver

Chunli Zhou

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Abstract

[Objective] The research aimed to compare the two extraction methods of genomic DNA from pork liver.[Method]kit method and chloroform/ isoamyl alcohol extraction method were adopted to extract genomic DNA from fresh pork liver.[Result] The results showed that the DNA extraction kit could obtain high purity genomic DNA with low density,and high concentration,good quality and relatively low cost by chloroform/ isoamyl alcohol method.[Conclusion] The chloroform/ isoamyl alcohol extraction of genomic DNA is a more effective method of promoting and using in molecular studies on fresh pork liver.

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What this paper is about

[Objective] The research aimed to compare the two extraction methods of genomic DNA from pork liver.[Method]kit method and chloroform/ isoamyl alcohol extraction method were adopted to extract genomic DNA from fresh pork liver.[Result] The results showed that the DNA extraction kit could obtain high purity genomic DNA with low density,and high concentration,good quality and relatively low cost by chloroform/ isoamyl alcohol method.[Conclusion] The chloroform/ isoamyl alcohol extraction of genomic DNA is a more effective method of promoting and using in molecular studies on fresh pork liver.

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Available abstract

[Objective] The research aimed to compare the two extraction methods of genomic DNA from pork liver.[Method]kit method and chloroform/ isoamyl alcohol extraction method were adopted to extract genomic DNA from fresh pork liver.[Result] The results showed that the DNA extraction kit could obtain high purity genomic DNA with low density,and high concentration,good quality and relatively low cost by chloroform/ isoamyl alcohol method.[Conclusion] The chloroform/ isoamyl alcohol extraction of genomic DNA is a more effective method of promoting and using in molecular studies on fresh pork liver.

Key concepts: Isoamyl alcohol, genomic DNA, DNA extraction, Chloroform, DNA, Extraction (chemistry), Alcohol, Chromatography

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