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Study on Rapid Propagation in vitro of Hosta plantaginea of Three New Species

Sun Wei-yi

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Abstract

The young buds of H.'Gloden Tiaia',H.'Gloden Gadet'and H.'Gloden Eagle'were used as the explants to develop a protocol for adventitious buds induced.The successful establishment of five species of rapid propagation system.The results showed that the best medium for adventitious buds induced was MS+6-BA 2.5 mg/L+ NAA 0.2 mg/L;In the proliferation culture,the best proliferation medium for H.'Gloden Tiaia'was MS+6-BA 2.0 mg/L+NAA 0.5 mg/L;The best proliferation medium for H.'Gloden Gadet'was MS+6-BA 1.0 mg/L+NAA 0.2 mg/L;The best proliferation medium for H.'Gloden Eagle'was MS+6-BA 4.0 mg/L+NAA 0.2 mg/L.Medium MS as H.'Gloden Eagle'rooting medium,the rooting rate was over 100%;H.'Gloden Gadet'in the rooting medium MS + NAA 0.5 mg/L;rooting rate was over 100%;H.'Gloden Tiaia'in the rooting medium MS + NAA 0.3 mg/L;rooting rate was over 100%.The transplant survival rate of 100%.

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What this paper is about

The young buds of H.'Gloden Tiaia',H.'Gloden Gadet'and H.'Gloden Eagle'were used as the explants to develop a protocol for adventitious buds induced.The successful establishment of five species of rapid propagation system.The results showed that the best medium for adventitious buds induced was MS+6-BA 2.5 mg/L+ NAA 0.2 mg/L;In the proliferation culture,the best proliferation medium for H.'Gloden Tiaia'was MS+6-BA 2.0 mg/L+NAA 0.5 mg/L;The best proliferation medium for H.'Gloden Gadet'was MS+6-BA 1.0 mg/L+NAA 0.2 mg/L;The best proliferation medium for H.'Gloden Eagle'was MS+6-BA 4.0 mg/L+NAA 0.2 mg/L.Medium MS as H.'Gloden Eagle'rooting medium,the rooting rate was over 100%;H.'Gloden Gadet'in the rooting medium MS + NAA 0.5 mg/L;rooting rate was over 100%;H.'Gloden Tiaia'in the rooting medium MS + NAA 0.3 mg/L;rooting rate was over 100%.The transplant survival rate of 100%.

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Available abstract

The young buds of H.'Gloden Tiaia',H.'Gloden Gadet'and H.'Gloden Eagle'were used as the explants to develop a protocol for adventitious buds induced.The successful establishment of five species of rapid propagation system.The results showed that the best medium for adventitious buds induced was MS+6-BA 2.5 mg/L+ NAA 0.2 mg/L;In the proliferation culture,the best proliferation medium for H.'Gloden Tiaia'was MS+6-BA 2.0 mg/L+NAA 0.5 mg/L;The best proliferation medium for H.'Gloden Gadet'was MS+6-BA 1.0 mg/L+NAA 0.2 mg/L;The best proliferation medium for H.'Gloden Eagle'was MS+6-BA 4.0 mg/L+NAA 0.2 mg/L.Medium MS as H.'Gloden Eagle'rooting medium,the rooting rate was over 100%;H.'Gloden Gadet'in the rooting medium MS + NAA 0.5 mg/L;rooting rate was over 100%;H.'Gloden Tiaia'in the rooting medium MS + NAA 0.3 mg/L;rooting rate was over 100%.The transplant survival rate of 100%.

Key concepts: Explant culture, Murashige and Skoog medium, In vitro, Botany, Biology, Horticulture, Chemistry, Biochemistry

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