Studies on the enzymatic hydrolysis techniques of preparing lamb placenta extract using Hu sheep placenta to replace Tibet sheep placenta

Objective To compare of the lamb placenta extracts(LPE) prepared by using Hu sheep plecenta and the traditional raw material(Tibet plecenta),So as to provide scientific evidence for using Hu sheep placenta instead of Tibet sheep placenta as the raw material preparing LPE.Methods With the Hu sheep placenta produced in the areas of Hangzhou,Jiaxing and Huzhou as raw material,and the Tibet sheep placenta,the traditional raw material as control,the optimal enzymatic hydrolysis experiments of preparing LPE were conducted.Results The optimal enzymatic parameters were as follows:enzymatic hydrolysis of placenta with 40 U/g tripsin at pH 8.0 and 45 ℃ for 4 h under the designed mixture of one material and ten liquid.Under the optimal condition,the degree of hydrolysis of Hu placenta and Tibet placenta were 17.56% and 19.44%,respectively,the both frozen LPE powder outputs were 35.36% and 40.47%,respectively,and the large molecular proteins of both placentas were degraded into LEP without significant difference in composition of amino acids.Conclusion Hu placenta is one raw material to replace Tibet placenta for preparing LPE.