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2010•Liaoning Zhongyiyao Daxue xuebaoRequires access

Determination of Sodium Danshensu in Xiaopining Granules by HPLC

Daizhou Zhang

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Abstract

Objective:To establish a method for determination of sodium danshensu in xiaopining granules by HPLC.Methods:Sodium danshensu content was determined on DiMa C18 Chromatographic column(200mm×4.6mm),with mobile phase of acetonitrile-water-acetic acid(5:160:1),Detected wavelength:281nm.Results:A good linear relationship was obtained when the detection concentration range was 0.1148～1.0332μg,and the average recovery is 100.24%,RSD is 1.23%.Conclusion:The method is fast and accurate,reproducible,high accuracy and the quality of preparation can be controlled.

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What this paper is about

Objective:To establish a method for determination of sodium danshensu in xiaopining granules by HPLC.Methods:Sodium danshensu content was determined on DiMa C18 Chromatographic column(200mm×4.6mm),with mobile phase of acetonitrile-water-acetic acid(5:160:1),Detected wavelength:281nm.Results:A good linear relationship was obtained when the detection concentration range was 0.1148～1.0332μg,and the average recovery is 100.24%,RSD is 1.23%.Conclusion:The method is fast and accurate,reproducible,high accuracy and the quality of preparation can be controlled.

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Available abstract

Objective:To establish a method for determination of sodium danshensu in xiaopining granules by HPLC.Methods:Sodium danshensu content was determined on DiMa C18 Chromatographic column(200mm×4.6mm),with mobile phase of acetonitrile-water-acetic acid(5:160:1),Detected wavelength:281nm.Results:A good linear relationship was obtained when the detection concentration range was 0.1148～1.0332μg,and the average recovery is 100.24%,RSD is 1.23%.Conclusion:The method is fast and accurate,reproducible,high accuracy and the quality of preparation can be controlled.

Key concepts: Medicine, Chromatography, High-performance liquid chromatography, Acetic acid, Sodium, Content determination, Linear range, Detection limit

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