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Determination of Danshensu in Tongjinglin Granules by HPLC

Xingping Luo, Wei Xue-bin

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Abstract

Subject: to determine the content of Danshensu in tongjinglin granules.Methods: The HPLC analysis was carried out on Kromasil C18 column(250 mm×4.6 mm,5 μm),the mixture of water-acetonitrile-acetic acid(93∶5∶2)was used as the mobile phase with the flow rate of 1.0 ml/min. The detection wavelength was at 280nm.Results: The linear range of Danshensu was 0.5～10 μg,the average recovery of this method was 97.55% with a RSD of 1.17%.Conclusion: The method can be applied to quantitative determination of the preparation.

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Subject: to determine the content of Danshensu in tongjinglin granules.Methods: The HPLC analysis was carried out on Kromasil C18 column(250 mm×4.6 mm,5 μm),the mixture of water-acetonitrile-acetic acid(93∶5∶2)was used as the mobile phase with the flow rate of 1.0 ml/min. The detection wavelength was at 280nm.Results: The linear range of Danshensu was 0.5～10 μg,the average recovery of this method was 97.55% with a RSD of 1.17%.Conclusion: The method can be applied to quantitative determination of the preparation.

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Available abstract

Subject: to determine the content of Danshensu in tongjinglin granules.Methods: The HPLC analysis was carried out on Kromasil C18 column(250 mm×4.6 mm,5 μm),the mixture of water-acetonitrile-acetic acid(93∶5∶2)was used as the mobile phase with the flow rate of 1.0 ml/min. The detection wavelength was at 280nm.Results: The linear range of Danshensu was 0.5～10 μg,the average recovery of this method was 97.55% with a RSD of 1.17%.Conclusion: The method can be applied to quantitative determination of the preparation.

Key concepts: Chromatography, High-performance liquid chromatography, Chemistry, Acetic acid, Content determination, Acetonitrile, Linear relationship, Linear range

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