2008Chinese Journal of Modern Drug ApplicationRequires access

Determination of Danshensu in Jiakangling Granules by HPLC

Gao Ya-xiong

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Abstract

Objective To determine Danshensu in Jiakangling Granules by HPLC.Methods HPLC analysis was applied to determine Danshensu in Jiakangling Granules.The HPLC chromatographic conditions included Hypersil BDS column;the mobile phase was methanol-1% glacial acetic(10:90)and the detective wavelength was at 280 nm,column temperature was 25℃.Results There were good linear relationship for Danshensu within the range of 3.612-18.06 μg/ml,r=0.999 9.The average recovery was 98.9%,RSD=1.7%(n=5).Conclusion The methods were of good accurate and a simple procedure.It may be practical to control the quality of Jiakangling Granules.

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What this paper is about

Objective To determine Danshensu in Jiakangling Granules by HPLC.Methods HPLC analysis was applied to determine Danshensu in Jiakangling Granules.The HPLC chromatographic conditions included Hypersil BDS column;the mobile phase was methanol-1% glacial acetic(10:90)and the detective wavelength was at 280 nm,column temperature was 25℃.Results There were good linear relationship for Danshensu within the range of 3.612-18.06 μg/ml,r=0.999 9.The average recovery was 98.9%,RSD=1.7%(n=5).Conclusion The methods were of good accurate and a simple procedure.It may be practical to control the quality of Jiakangling Granules.

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Available abstract

Objective To determine Danshensu in Jiakangling Granules by HPLC.Methods HPLC analysis was applied to determine Danshensu in Jiakangling Granules.The HPLC chromatographic conditions included Hypersil BDS column;the mobile phase was methanol-1% glacial acetic(10:90)and the detective wavelength was at 280 nm,column temperature was 25℃.Results There were good linear relationship for Danshensu within the range of 3.612-18.06 μg/ml,r=0.999 9.The average recovery was 98.9%,RSD=1.7%(n=5).Conclusion The methods were of good accurate and a simple procedure.It may be practical to control the quality of Jiakangling Granules.

Key concepts: Chromatography, High-performance liquid chromatography, Chemistry, Granule (geology), Materials science, Composite material

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