Culture Medium Filtrated of Grown Seeding Direct in Tissue Culture of Different Explants to Lonicera japonica var. Chinensis

Using the terminal bud,taking young stem and leaf of Lonicera japonica var.chinensis as experimental material,through the integrated analytic ways to culture seeding in form target,culture medium filtrated of fully grown seeding direct of three different explants to Lonicera japonica var.chinensis under the culture condition of imitating nature climate.The results showed that terminal bud was the best explant,the beter explant was leaf and young stem.The best adaptive culture medium to terminal bud MS+1.0 mg/L KT+1.0 mg/L 6-BA+0.3 mg/L α-NAA+30 g/L sucrose+8 g/L agar+0.3% activated carbon,pH 6.0;the best adaptive culture medium to young stem:MS+1.0 mg/L KT+1.0 mg/L 6-BA+0.1 mg/L α-NAA+30 g/L sucrose+8 g/L agar+0.3% activated carbon,pH 6.0;the best adaptive culture medium to leaf:MS+1.0mg/L KT+1.0mg/L 6-BA+0.3 mg/L α-NAA+30 g/L sucrose+8 g/L agar+0.3% activated carbon,pH 6.0.