2009•Journal of Tianjin Normal UniversityRequires access

The tissue culture method of Lanzhou Lily

Chengjin Guo

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Abstract

The optimal culture mediums were obtained by tissue culture method from Induction of bud,culture of multiplication and culture of root of taking Lanzhou Lily as a exophyte.They were:(1) the culture medium of bud induction: MS+BA 0.6 mg/L+NAA 0.2 mg/L+agar 7.3 g/L+sucrose 30 g/L,pH 5.8.(2) the culture medium of multiplication: MS+BA 0.8 mg/L+NAA 0.05mg/L+agar 7.3 g/L+sucrose 30 g/L,pH 5.8.(3) the culture medium of rooting culture: MS+BA 0.05 mg/L+NAA 0.8 mg/L+agar 7.3 g/L+sucrose 30 g/L,pH 5.8.Compared with existing ones,the multiplication rate of the former two ones were faster 15 days,the third culture medium was faster 2 days.

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The optimal culture mediums were obtained by tissue culture method from Induction of bud,culture of multiplication and culture of root of taking Lanzhou Lily as a exophyte.They were:(1) the culture medium of bud induction: MS+BA 0.6 mg/L+NAA 0.2 mg/L+agar 7.3 g/L+sucrose 30 g/L,pH 5.8.(2) the culture medium of multiplication: MS+BA 0.8 mg/L+NAA 0.05mg/L+agar 7.3 g/L+sucrose 30 g/L,pH 5.8.(3) the culture medium of rooting culture: MS+BA 0.05 mg/L+NAA 0.8 mg/L+agar 7.3 g/L+sucrose 30 g/L,pH 5.8.Compared with existing ones,the multiplication rate of the former two ones were faster 15 days,the third culture medium was faster 2 days.

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Available abstract

The optimal culture mediums were obtained by tissue culture method from Induction of bud,culture of multiplication and culture of root of taking Lanzhou Lily as a exophyte.They were:(1) the culture medium of bud induction: MS+BA 0.6 mg/L+NAA 0.2 mg/L+agar 7.3 g/L+sucrose 30 g/L,pH 5.8.(2) the culture medium of multiplication: MS+BA 0.8 mg/L+NAA 0.05mg/L+agar 7.3 g/L+sucrose 30 g/L,pH 5.8.(3) the culture medium of rooting culture: MS+BA 0.05 mg/L+NAA 0.8 mg/L+agar 7.3 g/L+sucrose 30 g/L,pH 5.8.Compared with existing ones,the multiplication rate of the former two ones were faster 15 days,the third culture medium was faster 2 days.

Key concepts: Agar, Sucrose, Tissue culture, Multiplication (music), Liquid culture, Botany, Biology, Horticulture

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