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Studies on Tissue Culture of Lonicera japonica var.chinensis

Xianhui Zhao

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Abstract

In this paper,terminal buds of Lonicera japonica var.chinensis were studied as the explants,through the orthogonal design.And this paper researched the preliminary culture、the subculture and the inducing root.The results showed that the best medium for the preliminary culture was MS+6-BA(1.0 mg/L)+NAA(0.1~0.2 mg/L),the best concentration of sucrose was 20.0 g/L;the best medium for the subculture was MS+6-BA(1.5 mg/L)+NAA(0.2 mg/L)+GA3(0.5 mg/L);the best medium for the root cultivated was 1/2MS+6-BA 0.25 mg/L+NAA 1.0 mg/L+AC 1.0 g/L.

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What this paper is about

In this paper,terminal buds of Lonicera japonica var.chinensis were studied as the explants,through the orthogonal design.And this paper researched the preliminary culture、the subculture and the inducing root.The results showed that the best medium for the preliminary culture was MS+6-BA(1.0 mg/L)+NAA(0.1~0.2 mg/L),the best concentration of sucrose was 20.0 g/L;the best medium for the subculture was MS+6-BA(1.5 mg/L)+NAA(0.2 mg/L)+GA3(0.5 mg/L);the best medium for the root cultivated was 1/2MS+6-BA 0.25 mg/L+NAA 1.0 mg/L+AC 1.0 g/L.

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Available abstract

In this paper,terminal buds of Lonicera japonica var.chinensis were studied as the explants,through the orthogonal design.And this paper researched the preliminary culture、the subculture and the inducing root.The results showed that the best medium for the preliminary culture was MS+6-BA(1.0 mg/L)+NAA(0.1~0.2 mg/L),the best concentration of sucrose was 20.0 g/L;the best medium for the subculture was MS+6-BA(1.5 mg/L)+NAA(0.2 mg/L)+GA3(0.5 mg/L);the best medium for the root cultivated was 1/2MS+6-BA 0.25 mg/L+NAA 1.0 mg/L+AC 1.0 g/L.

Key concepts: Subculture (biology), Japonica, Tissue culture, Sucrose, Explant culture, Botany, Horticulture, Biology

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