Intracellular ([Ca~(2+)]i) and the signal transducti on in Eca-109 cell apoptosis

Aim:To study the alteration of intracellular ([Ca 2+ ]i) in Eca 109 cell apoptosis and Ca 2+ participation into signal transduction.Methods:The cultured Eca 109 cells were randomly divided into 3 groups: 8 Br cAMP (Br)group, Quercetin (Q)group, and Control (C)group cultured with no any drugs. The 3 group cells were cultured simultaneously for 48h.The fluorescence intensity was detected with the laser confocal microscope. Results: The fluorescence intensity of intracellular ([Ca 2+ ]i) was 59.2±8.1 in C group and 63.3±8.5 in Br group, respectively, and there was no significant difference between the two groups ( P 0.05). However, the fluorescence intensity was 113.3±12.5 in Q group, which was much stronger than that in the C group( P 0.01).Conclusion: The alteration of intracellular ([Ca 2+ ]i) was different in cell apoptosis between Q and Br groups, suggesting that there was different pathway in apoptotic signal transduction between 8 Br cAMP and quercetin to a certain extent.