Ultrasound-assisted Extraction of Tanshinones from Analytical Samples of Salvia miltiorrhiza Bge.

An ultrasound-assisted extraction method for cryptotanshinone,tanshinone I and tanshinone IIA from analytical samples of Salvia miltiorrhiza Bge.was established.The optimal extraction conditions were obtained as follows:φ(CH_2Cl_2)=0.2 CH_3OH-CH_2Cl_2 as extracting solvents,8 min of extraction time and 133.3 mL/g of extracting solvent-to-sample ratio.Compared with conventional methods,such as methanol reflux extraction and CH_3OH-CH_2Cl_2 macerating at room temperature,the method of ultrasound-assisted extraction has the advantages of short extraction time and high efficiency for extracting tanshinones,which suggests that it is suitable for sample pretreatment in the simultaneous determination of cryptotanshinon,tanshinone I and tanshinone IIA in Salvia miltiorrhiza Bge..