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The Effect of Different Methods of Purification on the Quality of Total Flavones of Radix Puerariae

LI Huan-de, Luo Jie-ying

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Abstract

OBJECTIVE:To investigate the technique of purifying the total flavones of Radix Puerariae,so as to provide an experimental basis for extraction and purification of the flavones METHODS:Five purifying methods,including adsorption with macroreticular adsorption resin AB-8,D101,D201 and with polyamide,extration with n-butanol,were adopted,and the amount and purity of total flavones collected and preservation of the components of total flavones were compared RESULTS:By using the above-mentioned five methods,the percentages of the contents of total flavones were 89 4%,83 6%,91 0%,96 0% and 82 9% and the recoveries of products were 79 3%,64 2%,88 0%,55 7% and 77 2% respectively CONCLUSION:Among the five methods,adsorption with AB-8 macroreticular adsorption resin is the best,which may be used for purification of total flavones of Radix Puerariae

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OBJECTIVE:To investigate the technique of purifying the total flavones of Radix Puerariae,so as to provide an experimental basis for extraction and purification of the flavones METHODS:Five purifying methods,including adsorption with macroreticular adsorption resin AB-8,D101,D201 and with polyamide,extration with n-butanol,were adopted,and the amount and purity of total flavones collected and preservation of the components of total flavones were compared RESULTS:By using the above-mentioned five methods,the percentages of the contents of total flavones were 89 4%,83 6%,91 0%,96 0% and 82 9% and the recoveries of products were 79 3%,64 2%,88 0%,55 7% and 77 2% respectively CONCLUSION:Among the five methods,adsorption with AB-8 macroreticular adsorption resin is the best,which may be used for purification of total flavones of Radix Puerariae

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Available abstract

OBJECTIVE:To investigate the technique of purifying the total flavones of Radix Puerariae,so as to provide an experimental basis for extraction and purification of the flavones METHODS:Five purifying methods,including adsorption with macroreticular adsorption resin AB-8,D101,D201 and with polyamide,extration with n-butanol,were adopted,and the amount and purity of total flavones collected and preservation of the components of total flavones were compared RESULTS:By using the above-mentioned five methods,the percentages of the contents of total flavones were 89 4%,83 6%,91 0%,96 0% and 82 9% and the recoveries of products were 79 3%,64 2%,88 0%,55 7% and 77 2% respectively CONCLUSION:Among the five methods,adsorption with AB-8 macroreticular adsorption resin is the best,which may be used for purification of total flavones of Radix Puerariae

Key concepts: Flavones, Radix (gastropod), Chemistry, Chromatography, Extraction (chemistry), Adsorption, Organic chemistry, Botany

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