Impact of Physical and Chemical Characteristics of Resin on Separating Total Flavones from Radix Puerariae Lobatae

OBJECTIVE Study on the impact of the physical and chemical characteristics of resin on separating total flavones from Radix Puerariae Lobatae.METHODS Five kinds of ADS adsorbing resins were tested in the static and dynamic research about separating and purifying total flavones.And the best resin was screened out,then its technical parameters in dynamic adsorption-desorption were optimized.RESULTS The static adsorption tests showed that the polarity and textual structure were main factors on the adsorptive capacity.The optimum conditions for ADS-8 were identified,which were 0.453 mg·mL-1 corresponding to crude drug for concentration of extract,pH 5-6 with the adsorption speed of 4 BV·h-1;water volume for removing impurities was 400 mL;and the concentration of the eluant ethanol 70%,volume of ethanol was 60 mL with the desorption speed of 2 BV·h-1.CONCLUSION ADS-8 is the best resin for separating and purifying total flavones.