Comparative studies on different methods of purification of total flavones from Mulberry leaf

Objective To compare the purification effects of different purification techniques of total flavones from Mulberry leaf.In order to find a purification technology which is cheap,simple,feasible and suitable for the production of total flavones whose purity is more than 15%.Methods Six purifying methods,including adsorption with macroreticular adsorption resin D101,AB-8,JD-1,extraction with ethylacetate,alcohol sedimentation method,alcohol sedimentation and alcohol precipitation method,were adopted,and the amound and purity of total flavones collected were compared.Results By using the above-mentioned six methods,the percentages of the contents of total flavones was 50.3%,48.5%,55.7%,82.1%, 10.8% and 18.5% respectively,the recoveries of products was 2.37%,2.42%,2.28%,0.45%,18.21% and 15.93% respectively.Conclusion Using the alcohol sedimentation and alcohol precipitation method can get total flavones up to requirement.