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2010•China Modern MedicineRequires access

Determination of puerarin in Yangyin Jiangtang Capsule by HPLC

Lin Mingyue

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Abstract

Objective: To determine the contents of the puerarin in Yangyin Jiangtang Capsule by HPLC. Methods: HPLC was used with a column of Agilent XDB C18 (4.6 mm×250 mm, 5 μm) and a mobile phase of methanol-phosphate buffer (20:80), the flow rate was 1.0 ml/min, the detection wavelength was 250 nm and the injection volume was 10 μl. Results: Puerrarin in the range of 0.100～3.100 μg good linear relationship (r=0.999 6), the average recovery was 98.46%, RSD was 0.80%(n=5). Conclusion: The method is simple, reliable, specific, Yin Capsule as the determination of puerarin in order to effectively control the content of the preparation.

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What this paper is about

Objective: To determine the contents of the puerarin in Yangyin Jiangtang Capsule by HPLC. Methods: HPLC was used with a column of Agilent XDB C18 (4.6 mm×250 mm, 5 μm) and a mobile phase of methanol-phosphate buffer (20:80), the flow rate was 1.0 ml/min, the detection wavelength was 250 nm and the injection volume was 10 μl. Results: Puerrarin in the range of 0.100～3.100 μg good linear relationship (r=0.999 6), the average recovery was 98.46%, RSD was 0.80%(n=5). Conclusion: The method is simple, reliable, specific, Yin Capsule as the determination of puerarin in order to effectively control the content of the preparation.

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Available abstract

Objective: To determine the contents of the puerarin in Yangyin Jiangtang Capsule by HPLC. Methods: HPLC was used with a column of Agilent XDB C18 (4.6 mm×250 mm, 5 μm) and a mobile phase of methanol-phosphate buffer (20:80), the flow rate was 1.0 ml/min, the detection wavelength was 250 nm and the injection volume was 10 μl. Results: Puerrarin in the range of 0.100～3.100 μg good linear relationship (r=0.999 6), the average recovery was 98.46%, RSD was 0.80%(n=5). Conclusion: The method is simple, reliable, specific, Yin Capsule as the determination of puerarin in order to effectively control the content of the preparation.

Key concepts: Puerarin, Medicine, Capsule, High-performance liquid chromatography, Chromatography, Content determination, Pathology, Chemistry

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