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2009•Qilu Pharmaceutical AffairsRequires access

Determination of puerarin in Shiwei Jiangtang Capsule by HPLC

Kang Ying-ying

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Abstract

OBJECTIVETo determine the contents of the puerarin in Shiwei Jiangtang Capsule by HPLC.METHODSRP-HPLC was used with a column of Agilent XDB C18(250mm×4.6mm,5μm) and a mobile phase of methanol-phosphate buffer(20∶80),the flow rate was 1mL·min-1,the detection wavelength was 250nm and the column temperature was 25℃,the injection volume was 10μL.RESULTSThe retention time of the puerarin was about 27min,the resolution factor between puerarin and adjacent peak is not less than 2.0.Good linear correlation existed,when the puerarin was within 0.024~0.476μg.The correlation coefficient was 0.9999.The average recoveries of paeoniflorin were 98.81%(n=5).CONCLUSIONThis method was accurate,reliable and can be used to control puerarin in Shiwei Jiangtang Capsule.

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OBJECTIVETo determine the contents of the puerarin in Shiwei Jiangtang Capsule by HPLC.METHODSRP-HPLC was used with a column of Agilent XDB C18(250mm×4.6mm,5μm) and a mobile phase of methanol-phosphate buffer(20∶80),the flow rate was 1mL·min-1,the detection wavelength was 250nm and the column temperature was 25℃,the injection volume was 10μL.RESULTSThe retention time of the puerarin was about 27min,the resolution factor between puerarin and adjacent peak is not less than 2.0.Good linear correlation existed,when the puerarin was within 0.024~0.476μg.The correlation coefficient was 0.9999.The average recoveries of paeoniflorin were 98.81%(n=5).CONCLUSIONThis method was accurate,reliable and can be used to control puerarin in Shiwei Jiangtang Capsule.

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Available abstract

OBJECTIVETo determine the contents of the puerarin in Shiwei Jiangtang Capsule by HPLC.METHODSRP-HPLC was used with a column of Agilent XDB C18(250mm×4.6mm,5μm) and a mobile phase of methanol-phosphate buffer(20∶80),the flow rate was 1mL·min-1,the detection wavelength was 250nm and the column temperature was 25℃,the injection volume was 10μL.RESULTSThe retention time of the puerarin was about 27min,the resolution factor between puerarin and adjacent peak is not less than 2.0.Good linear correlation existed,when the puerarin was within 0.024~0.476μg.The correlation coefficient was 0.9999.The average recoveries of paeoniflorin were 98.81%(n=5).CONCLUSIONThis method was accurate,reliable and can be used to control puerarin in Shiwei Jiangtang Capsule.

Key concepts: Puerarin, Chromatography, Chemistry, High-performance liquid chromatography, Paeoniflorin, Capsule, Correlation coefficient, Linear correlation

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