2008•Traditional Chinese Drug Research and Clinical PharmacologyRequires access
Determination of Puerarin in Huanglian Jiangtang Tablets by HPLC
Zhang Shudan
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Abstract
Objective To establish a method for the determination of Puerarin in Huanglian Jiangtang Tablets. Methods The sample was extracted with 30 %ethanol. The chromatographic conditions were as follows:Diamonsil C18 chromatographic column(250 mm×4.6 mm,5μm)with a mobile phase of acetonitrile-0.5 %glacial acetic acid,the detection wavelength being at 250 nm and the flow rate being 1.0 mL·min-1. Results A linearity was obtained from 0.338 μg to 2.336 μg of Puerarin in Huanglian Jiangtang Tablets with a good correlation (r=0.999997,n=7).The average recovery was 100.2% and RSD=1.80%(n=6). Conclusion This method for determination of Puerarin in Huanglian Jiangtang Tablets is easy,sensitive,specific and accurate.