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2003•Yunnan Forestry Science and TechnologyRequires access

Study on Tissue Culture of Stem Apex of Azadirachta indica

Fang Chen

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Abstract

The experiment of in vitro propagation of Azadirachta indica using the stem apex as explant was carried out. The explant was pre treated by sodium hypochlorite, soaking with HgCl 2 of 0 1 % in order to realize sterilization. Sixty treatments, and three replications were set up. The experimental results showed that the optimum preparations of bud enrichment and rooting were MS + 6-BA 7 0 mg/L + IAA 0 01 mg/L + ZT 0 1mg/L and 1/2 MS + NAA 0 5 mg/L respectively. The survival ratio of transplantation could achieve 80 %.

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What this paper is about

The experiment of in vitro propagation of Azadirachta indica using the stem apex as explant was carried out. The explant was pre treated by sodium hypochlorite, soaking with HgCl 2 of 0 1 % in order to realize sterilization. Sixty treatments, and three replications were set up. The experimental results showed that the optimum preparations of bud enrichment and rooting were MS + 6-BA 7 0 mg/L + IAA 0 01 mg/L + ZT 0 1mg/L and 1/2 MS + NAA 0 5 mg/L respectively. The survival ratio of transplantation could achieve 80 %.

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Available abstract

The experiment of in vitro propagation of Azadirachta indica using the stem apex as explant was carried out. The explant was pre treated by sodium hypochlorite, soaking with HgCl 2 of 0 1 % in order to realize sterilization. Sixty treatments, and three replications were set up. The experimental results showed that the optimum preparations of bud enrichment and rooting were MS + 6-BA 7 0 mg/L + IAA 0 01 mg/L + ZT 0 1mg/L and 1/2 MS + NAA 0 5 mg/L respectively. The survival ratio of transplantation could achieve 80 %.

Key concepts: Azadirachta, Explant culture, Apex (geometry), Sodium hypochlorite, Sterilization (economics), Horticulture, Biology, Tissue culture

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