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2004•Xi'nan Nongye Daxue xuebaoRequires access

TISSUE CULTURE AND RAPID PROPAGATION OF AZADIRACHTA INDICA A. JUSS

Wei Gong

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Abstract

Stem segments with buds from Azadirachta indica A. Juss were used as explants in tissue culture for rapid propagation. A satisfactory procedure was established, with which a 100% of callus induction and a multiplication rate of 5～7 times were achieved in 30 (days′) culture. The optimum media were MS + BA 1.0 mg/L + NAA 0.01 mg/L + 3% sucrose for callus induction, MS + BA 0.5 mg/L + NAA 0.1 mg/L + 3% sucrose for subculture, and 1/2 MS + NAA 0.01 mg/L + 1.5% sucrose for rooting induction.

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What this paper is about

Stem segments with buds from Azadirachta indica A. Juss were used as explants in tissue culture for rapid propagation. A satisfactory procedure was established, with which a 100% of callus induction and a multiplication rate of 5～7 times were achieved in 30 (days′) culture. The optimum media were MS + BA 1.0 mg/L + NAA 0.01 mg/L + 3% sucrose for callus induction, MS + BA 0.5 mg/L + NAA 0.1 mg/L + 3% sucrose for subculture, and 1/2 MS + NAA 0.01 mg/L + 1.5% sucrose for rooting induction.

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Available abstract

Stem segments with buds from Azadirachta indica A. Juss were used as explants in tissue culture for rapid propagation. A satisfactory procedure was established, with which a 100% of callus induction and a multiplication rate of 5～7 times were achieved in 30 (days′) culture. The optimum media were MS + BA 1.0 mg/L + NAA 0.01 mg/L + 3% sucrose for callus induction, MS + BA 0.5 mg/L + NAA 0.1 mg/L + 3% sucrose for subculture, and 1/2 MS + NAA 0.01 mg/L + 1.5% sucrose for rooting induction.

Key concepts: Subculture (biology), Azadirachta, Callus, Explant culture, Sucrose, Tissue culture, Botany, Horticulture

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