Establishment of Regeneration System for High Frequency Plantlet of Azadirachta indica A. Juss

[Objective] The study aimed to establish the regeneration system for high frequency plantlets of Azadirachta indica A.Juss.[Method] Taking A.indica stem segments with buds as the tested explants,MS as the basic medium,14 treatments of medium formulas were matched by adding 6-BA 0.1,0.3,0.5,0.8,1.0 mg/L and NAA 0,0.1,0.3,0.5mg/L to make the culture test of inducing the bud differentiation.3 kinds of media of MS+IBA 0.2 mg/L,1/2MS and MS were used to make the rooting culture.The optimum sanitizing time and the material parts for the explants were studied and the optimum medium formulas for inducing the bud differentiation and rooting of A.indica were screened.[Result] In initial culture,the optimum sanitizing time for the explants of A.indica was 10-12 min and the optimum material parts were shoot tip and the top of the plant.Among 14 medium treatments,the optimum medium for bud differentiation was MS+ 0.5 mg/L 6-BA+ 0.1 mg/L NAA + 3% sucrose(pH 5.8) + 0.65% agar and the optimum rooting medium was 1/2 MS medium + 3% sucrose + 0.65% agar(pH 5.8),with rooting rate of 85.13% and the survival rate of 80% for the transplanted plantlets.[Conclusion] This study laid the foundation for the massive micro-propagation and genetic transformation of A.indica.