Protective effects of "Ronggan Mixture" on immunological chronic liver injury in mice

Objective To investigate the protective effects of Ronggan Mixture on immunological chronic liver injury in mice induced by concanavalin A(ConA)and its mechanisms.Methods HBV transgenic mice were randomly divided into normal group(10)and ConA model group(59).The normal group was given PBS by the tail vein injection(0.3 ml),while the others were given ConA by the tail vein injection(6 μg/g);8 weeks after the modeling,the ConA-induced mice were devided into model group,Ronggan Mixture group,Yinchen group,Yinchenhao Decotion group and Bifendate group.Then the mice were gastrically perfused with corresponding therapeutic interventions for 4 weeks,while the normal group and model group were given normal saline.Twenty-four hours after the last gastric perfusion,the mice were sacrificed,then the serum and tissue samples were collectd to detect various indicators:ALT,AST,TBIL,SOD,MDA,TNF-α,INF-γ,IL-4 and IL-10.Results Significant differences were found in all indicators between normal group and model group(P0.05 or P0.01).Compared with the model group,the indicators of ALT,AST and TBIL were significanly lower in Ronggan Mixture group and Bifendate group(P0.01);the level of SOD in the liver increased after the intervention of the Ronggan Mixture,Yinchenhao Decotion and Bifendate(P0.01);while Ronggan Mixture and Yinchen solution could decrease the level of MDA(P0.05);Ronggan Mixture could decrease the level of TNF-α and IFN-γ,and increase the level of IL-4 and IL-10 in the liver tissue(P0.01 or P0.05).Meanwhile,the effect of lowering ALT and AST in Ronggan Mixture group was superior to Yinchenhao Decotion group and Yinchen group(P0.05);Ronggan Mixture had better effect in increasing SOD than Yinchenhao Decotion,Yinchen solution and Bifendate(P0.01);compared with Yinchen group and Bifendate group,Ronggan Mixture could obviously lower the expression of IFN-γ in liver tissue(P0.05 or P0.01).Conclusion The Ronggan Mixture has protective effect on chronic immunological liver injury in mice induced by ConA by blocking the lipid peroxidation of hepatocyte and regulating the balance of Th1/Th2 ceu factors level.